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human tracheobronchial epithelial htbe cells  (ATCC)


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    ATCC human tracheobronchial epithelial htbe cells
    Human Tracheobronchial Epithelial Htbe Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tracheobronchial epithelial htbe cells/product/ATCC
    Average 99 stars, based on 519 article reviews
    human tracheobronchial epithelial htbe cells - by Bioz Stars, 2026-03
    99/100 stars

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    Growth in HTBE cells revealed attenuation of certain reassortant genotypes. Triplicate wells of fully differentiated HTBE cells were inoculated with the indicated viruses at an MOI of 0.001 PFU/cell and incubated at 33°C. Viral titers present in apical samples collected at the indicated time points were determined by a plaque assay on MDCK cells. The mean result of three replicates is plotted, with the standard deviation indicated by an error bar. (A) Growth of viruses with H3N2-derived PB2 in HTBE cells; (B) growth of viruses with pH1N1-derived PB2 in HTBE cells.

    Journal: Journal of Virology

    Article Title: Seasonal H3N2 and 2009 Pandemic H1N1 Influenza A Viruses Reassort Efficiently but Produce Attenuated Progeny

    doi: 10.1128/JVI.00830-17

    Figure Lengend Snippet: Growth in HTBE cells revealed attenuation of certain reassortant genotypes. Triplicate wells of fully differentiated HTBE cells were inoculated with the indicated viruses at an MOI of 0.001 PFU/cell and incubated at 33°C. Viral titers present in apical samples collected at the indicated time points were determined by a plaque assay on MDCK cells. The mean result of three replicates is plotted, with the standard deviation indicated by an error bar. (A) Growth of viruses with H3N2-derived PB2 in HTBE cells; (B) growth of viruses with pH1N1-derived PB2 in HTBE cells.

    Article Snippet: Human tracheobronchial epithelial (HTBE) cells from a single donor were acquired from Lonza and were amplified and differentiated into air-liquid interface cultures as recommended by Lonza and described by Danzy et al. ( 79 ).

    Techniques: Incubation, Plaque Assay, Standard Deviation, Derivative Assay